The Biological Significance of the Lipid Bilayer’s Melting Transition

Dr.Martin Bier

East Carolina
UniversityDepartment of Physics

Wednesday, 16th September 2009
3:00PM Samsung Auditorium

At the temperature of the gel-liquid phase transition, a lipid bilayer membrane exhibits an increased ion permeability. The increased permeability presents itself in the form of quantized currents. It is surprising that pure lipid bilayers can actually behave like ion channels. A standard analysis of measured currents will be shown. The open time histogram exhibits a “-3/2” power law which implies a nonmarkovian open-closed transition rate that decreases like k(t) / t?1 as time evolves. A “pore freezing” model will be proposed to explain the observations. This model also leads to the 1/f noise that is commonly observed in currents across biological and artificial membranes. It has been proposed that an action potential going through a nerve cell is not merely an electro-chemical phenomenon, but also involves a traveling wave of compression and partial freezing of the lipid bilayer membrane. We have performed experiments that are intended to discriminate between the electro-chemical and the thermodynamic mechanism. We found that a nerve that is affected by an anesthetic can nevertheless reach the same compound action potential as an unaffected nerve when it receives a higher stimulus voltage. The result is hard to reconcile with the electro-chemical Hodgkin-Huxley model and consistent with the thermodynamic mechanism. 1